Coding

Part:BBa_K1510011:Experience

Designed by: Chien-Yung Huang   Group: iGEM14_NYMU-Taipei   (2014-10-06)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1510011

Adding endolysin specific to oral bactria, Streptococcus Mutans (S.Mutans), this part can be applicated in oral hygiene technique.

User Reviews

UNIQc8fcaa4e2f0fc260-partinfo-00000000-QINU

By doing disc plate assay of endolysin together with several other controls, we can see that there is a clear space besides the disc of our different concentration of our endolysin supernatant.

As shown in the figure, ampicillin showed a significant inhibition zone as a positive control. Three positive control all show there ability to kill Streptococcus Mutans by the clear zone around the disc. The BHI negative control showed no killing ability as expected. However, there are also little Streptococcus Mutans growing near J23100+yebF+B0015 culture supernatant. By observing the pattern on the plate, we speculated that it might be the result of the diffusion of Ampicillin beside it.

NYMU chienyunghuang endolysin function test.jpg UNIQc8fcaa4e2f0fc260-partinfo-00000001-QINU